Induction and Quantification of Neutrophil Extracellular Traps
作者： Alejandro Sanchez Gonzalez , Bart W. Bardoel , Christopher J. Harbort , Arturo Zychlinsky
DOI: 10.1007/978-1-62703-845-4_20
关键词: Quantification methods 、 Cell biology 、 Innate immune system 、 Neutrophil extracellular traps 、 Peripheral blood 、 Chemistry
摘要: Neutrophil extracellular trap (NET) formation is a recently discovered process in the field of innate immunity. It important to have consistent standards inducing and quantifying NET compare data from different labs this new area investigation. Here we describe conditions neutrophil isolation peripheral blood stimulation that find allow study NETosis vitro. The criteria for conclusively identifying NETosis, pros cons various quantification methods are discussed.
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M Monestier, T M Fasy, K E Novick, M J Losman, Monoclonal autoantibodies to subnucleosomes from a MRL/Mp(-)+/+ mouse. Oligoclonality of the antibody response and recognition of a determinant composed of histones H2A, H2B, and DNA. Journal of Immunology. ,vol. 148, pp. 1561- 1569 ,(1992)
Kathleen D. Metzler, Tobias A. Fuchs, William M. Nauseef, Dominique Reumaux, Joachim Roesler, Ilka Schulze, Volker Wahn, Venizelos Papayannopoulos, Arturo Zychlinsky, Myeloperoxidase is required for neutrophil extracellular trap formation: implications for innate immunity. Blood. ,vol. 117, pp. 953- 959 ,(2011) , 10.1182/BLOOD-2010-06-290171
A. Hakkim, B. G. Furnrohr, K. Amann, B. Laube, U. A. Abed, V. Brinkmann, M. Herrmann, R. E. Voll, A. Zychlinsky, Impairment of neutrophil extracellular trap degradation is associated with lupus nephritis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 9813- 9818 ,(2010) , 10.1073/PNAS.0909927107
Volker Brinkmann, Christian Goosmann, Lars I. Kühn, Arturo Zychlinsky, Automatic quantification of in vitro NET formation Frontiers in Immunology. ,vol. 3, pp. 413- 413 ,(2013) , 10.3389/FIMMU.2012.00413
Volker Brinkmann, Ulrike Reichard, Christian Goosmann, Beatrix Fauler, Yvonne Uhlemann, David S Weiss, Yvette Weinrauch, Arturo Zychlinsky, Neutrophil extracellular traps kill bacteria Science. ,vol. 303, pp. 1532- 1535 ,(2004) , 10.1126/SCIENCE.1092385
Venizelos Papayannopoulos, Kathleen D. Metzler, Abdul Hakkim, Arturo Zychlinsky, Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps Journal of Cell Biology. ,vol. 191, pp. 677- 691 ,(2010) , 10.1083/JCB.201006052
R. Lande, D. Ganguly, V. Facchinetti, L. Frasca, C. Conrad, J. Gregorio, S. Meller, G. Chamilos, R. Sebasigari, V. Riccieri, R. Bassett, H. Amuro, S. Fukuhara, T. Ito, Y.-J. Liu, M. Gilliet, Neutrophils Activate Plasmacytoid Dendritic Cells by Releasing Self-DNA–Peptide Complexes in Systemic Lupus Erythematosus Science Translational Medicine. ,vol. 3, ,(2011) , 10.1126/SCITRANSLMED.3001180