Effect of Propionic and Methylmalonic Acids on the High Molecular Weight Neurofilament Subunit (NF-H) in Rat Cerebral Cortex
作者： L. Vivian , F. Dall Bello Pessutto , L. M. Vieira de Almeida , S. de Oliveira Loureiro , P. de Lima Pelaez
关键词: Chemistry 、 Phosphorylation 、 Neurofilament 、 Methylmalonic acid 、 Dephosphorylation 、 Molecular biology 、 Cytoskeleton 、 Cerebral cortex 、 Specific antibody 、 Biochemistry 、 Protein subunit
摘要: Propionic and methylmalonic acidemias are inherited neurometabolic disorders biochemically characterized by tissue accumulation of propionic (PA) (MMA) acids, respectively. Neurofilaments (NF) important cytoskeletal proteins phosphorylation/dephosphorylation NF is to stabilize the cytoskeleton. We investigated effects PA MMA on high molecular weight neurofilament subunit associated with fraction rat cerebral cortex along development. Cortical slices from 9- 60-day-old rats were incubated 2.5 mM or MMA. The was extracted immunoreactivity for phosphorylated total NF-H analyzed immunoblotting using specific antibodies. Results showed that treatment acids induced an increased Triton-insoluble in up 17-day-old rats. Furthermore, treatments significantly amount 12-day-old These findings indicate alter dynamic regulation assembly fraction.
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