Limits of detection of nuclear DNA abnormalities by flow cytometric DNA analysis. Results obtained by a set of methods for sample‐storage, staining and internal standardization

摘要: Flow cytometric DNA analysis is an attractive alternative to conventional cytogenetics for diagnosing karyotype changes resulting in abnormal content. We have developed methods long-term storage of samples and standards, staining the cells standardization measurements by two internal standards. In this paper currently attainable resolution was determined limiting factors were identified. Normal reference values male female leucocytes analyzing 240 from six men women, all cytogenetically normal. The content 1.5% higher than that cells. This finding used correct results sex related differences. 95% confidence limits mononuclear blood ±0.79%. on granulocytes exceptionally variable, with ±1.79%. Reexamination normal after 1 year showed a drift up 2% results, indicating need regular checks values. addition differences content, individual 1% demonstrated. creates problems as which value use particular sample. Furthermore, tissue fluorescence found when different tissues one mouse examined. means had range 0.7%. add uncertainty interpretation results. heterogeneous populations slightly examined mixtures known ranging 1.50% 5.83%. With coefficient variation peaks about difference approximately 4% required accurate determination populations. A lower would increase resolution, but could then become factor.