Disturbance of chiral ionic liquids to phototaxis of Chlamydomonas reinhardtii: regular analysis and mechanism attempt.

摘要: Given the recent extensive synthesis and application of ionic liquids (ILs), finding a sensitive visual indicator to provide fast-initial risk assessment IL use has become pressing issue. In this study, we verified that phototaxis Chlamydomonas reinhardtii is valid environmental associated with chiral ILs L-(+)- D-(-)-1-butyl-3-methylimidazolium lactate (BMIM L). Briefly, C. was exposed 4000-lx side light source for varying lengths time. Following allotted exposure time, algae aggregation photographed, then quantitative analysis conducted using Image-J software obtain corresponding relationship between stimulation phototaxis. The gray areas from each treatment were measured percentage calculated. After 16 h lighting, control, - 22%, while D-(-)- BMIM L 17% 33%, respectively. Then, after 8 h darkness, where D-(-)-BMIM control showed positive phototaxis, but L-(+)-BMIM L-treated group virtually no change. This phenomenon consistent excessive production reactive oxygen species (ROS). Moreover, atomic force microscope (AFM) results indicated distinct L, which caused changes in cell permeability induced change ROS transfer. Furthermore, ultrastructure photosynthetic efficiency also investigated. work demonstrates potential serve as sensitive, convenient, cost effective qualitative ILs' toxic impact, understanding evaluation requires further improvement.