Polyphenolic Compounds Extracted and Purified from Buddleja Globosa Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography.

摘要: Chemical profiling of Buddleja globosa was performed by high-performance liquid chromatography coupled to electrospray ionization (HPLC-DAD-ESI-IT/MS) and quadrupole time-of-flight high-resolution mass spectrometry (HPLC-ESI-QTOF/MS). The identification 17 main phenolic compounds in B. leaf extracts achieved. Along with caffeoyl glucoside isomers, caffeoylshikimic acid several verbascoside derivatives (β-hydroxyverbascoside β-hydroxyisoverbascoside) were identified. Among flavonoid compounds, the presence 6-hydroxyluteolin-7-O-glucoside, quercetin-3-O-glucoside, luteolin 7-O-glucoside, apigenin 7-O-glucoside confirmed. Campneoside I, forsythoside B, lipedoside A identified along verbascoside, isoverbascoside, eukovoside martynoside. isolation two bioactive B from (Buddlejaceae) successfully achieved centrifugal partition (CPC). Both obtained one-step using optimized CPC methodology two-phase solvent system comprising ethyl acetate-n-butanol-ethanol-water (0.25:0.75:0.1:1, v/v). Additionally, eight Natural Deep Eutectic Solvents (NADESs) tested for extraction polyphenols compared 80% methanol. contents after methanol 26.165 3.206 mg/g, respectively. NADESs this study, proline- citric (1:1) choline chloride-1, 2- propanediol (1:2) most promising solvents. With these NADES, yields 51.045 4.387 Taken together, results study confirm that enabled fast globosa. displayed higher efficiency therefore could be used as an ecofriendly alternative classic organic