Detection and quantification of 14 Campylobacter species in pet dogs reveals an increase in species richness in feces of diarrheic animals

摘要: Background: The genus Campylobacter includes many species, some of which are known human and animal pathogens. Even though studies have repeatedly identified domestic dogs as a risk factor for campylobacteriosis, our understanding ecology in this reservoir is limited. Work to date has focused primarily on limited number species using culture-based methods. To expand dogs, collection fecal samples from 70 healthy 65 diarrheic pet were examined the presence levels 14 quantitative PCR. Results: It was found that 58% 97% shed detectable spp., with C. coli, concisus, fetus, gracilis, helveticus, jejuni, lari, mucosalis, showae, sputorum upsaliensis significantly higher population. Levels individual detected ranged 10 3 8 organisms per gram feces. In addition, contained multiple Campylobacter, carrying 0-7 while carried 0-12 species. Conclusions: These findings represent largest specifically tested animals first report determine quantifiable being dogs. This study demonstrates can carry wide range naturally there notable increase richness With several or emerging pathogens, these results relevant both ecological public health discussions.