mRNA precursor splicing in vivo: sequence requirements determined by deletion analysis of an intervening sequence

摘要: To define the extent of intervening sequence required for splicing higher eukaryotic mRNA precursors in vivo, we constructed deletions within second human G gamma-globin gene that progressively approach donor or acceptor splice sites. Most can be deleted with no effect on splicing. At site, 6 bases are sufficient accurate and efficient 20 splicing, 16 but at a reduced level. However, 15 insufficient significant The ending near site is independent whether an A-G dinucleotide introduced into region by deletion.