Naturally occurring genetic markers in lactobacilli and their use to verify the authenticity of Swiss Emmental PDO cheese
作者： Michael G. Casey , Dino Isolini , Rudolf Amrein , Daniel Wechsler , Hélène Berthoud
DOI: 10.1051/DST:2008014
关键词: Microbiology 、 Amplicon 、 Bacterial genome size 、 Genetics 、 Emmental cheese 、 Swiss cheese 、 Genetic marker 、 Genome 、 Biology 、 Lactobacillus 、 Insertion sequence
摘要: A general method for the detection and identification of specific strains bacteria is described. The assay based on observation that insertion sequences (IS) in different occur at diverse loci bacterial genome. Exclusive PCR primers can be selected a particular strain where one IS element other adjacent DNA sequence Only containing point genome will yield an amplicon expected size after PCR. We have illustrated this by selecting to detect some lactobacilli are used exclusively manufacture Swiss Emmental cheese. Using we were able differentiate cheeses manufactured Switzerland from those made European countries. transposase / strain-specific lactic acid
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