Genistein Triggers Translocation of Estrogen Receptor-Alpha in Mitochondria to Induce Expressions of ATP Synthesis-Associated Genes and Improves Energy Production and Osteoblast Maturation.

摘要: Our previous study showed that estrogen can induce mitochondrial adenosine triphosphate (ATP) synthesis-associated gene expressions and osteoblast maturation. Genistein, a phytoestrogenic isoflavone is widely found in various foods traditional herb products, beneficial for osteogenesis by selectively triggering receptor alpha (ER[Formula: see text] expression. In this study, we further investigated the mechanisms of genistein-induced energy production activation. Exposure rat calvarial osteoblasts human U-2 OS cells to genistein triggered activation without affecting cell survival. Treatment with time-dependently induced ER[Formula: mRNA protein osteoblasts. Analyses confocal microscopy immunoblotting stimulated translocation from cytoplasm mitochondria. Subsequently, cytochrome c oxidase (COX) I II mRNAs proteins primary were after exposure genistein. Knocking-down concurrently inhibited COX expressions. addition, complex enzyme activities, membrane potential, cellular ATP levels augmented Suppressing expression instantaneously lowered enhancements Effects on translocation, expressions, synthesis, confirmed cells. This stimulate consequent via inducing text]-mediated synthesis-linked