Amebic infections due to the Entamoeba histolytica-Entamoeba dispar complex: a study of the incidence in a remote rural area of Ecuador.

摘要: An epidemiologic field study was conducted in the village of Borbon Esmeraldas province northern Ecuador to compare different parasitologic methods diagnosis infection with Entamoeba histolytica/Entamoeba dispar complex. The results two stool antigen detection assays (the Prospect histolytica microplate assay and E. II assay) were compared isoenzyme characterization amebic isolates. Nearly all (176 178, 98.9%) subjects positive for intestinal parasites on direct microscopic examination, cysts and/or vegetative forms morphologically consistent histolytica/E. complex recorded 48 178 cases (27%). Culture Robinson's medium stocks 89 (50%) samples tested. Of 37 isolates successfully stabilized, cloned, characterized by zymodeme analysis, seven (18.9%) showed patterns histolytica, whereas 26 (70.3%) dispar. remaining four strains identified as coli (three isolates; 8.1%) Dientamoeba fragilis (one strain; 2.7%).The immunochromatographic tests degrees sensitivity specificity when reference technique. assay, which does not discriminate between E.dispar, a 54.5% 94% both these species. In contrast, second-generation test had 14.3% 98.4% sensu stricto. Our survey clearly demonstrated that more specific sensitive diagnostic tests, such are needed establish actual worldwide distribution