DNA typing with fluorescently tagged short tandem repeats: a sensitive and accurate approach to human identification.

摘要: Human identification through DNA analysis has faced tremendous changes in the past seven years. The advent of polymerase chain reaction (PCR) technology coupled with discovery amplifiable minisatellites and microsatellites known as amplified fragment length polymorphisms short tandem repeats (STRs), respectively, allow allelic profiles to be obtained minute amounts target even a degraded state. Very recently, new dimension typing was opened development instruments for automated real-time fluorescent amplification products. In order derive an approach typing, STR systems were evaluated sensitivity accuracy using Gene Scanner compared other methods currently use. Eight different (encompassing tri-, tetra- pentanucleotide repeats) investigated, conditions their fluorescence-tagged primers, resolution on polyacrylamide gels analyzer optimized. Using these conditions, discrete following extracted from various cell lines, liquid blood, dry bloodstains hair samples. Amplification serial dilutions template indicated that minimal amount required detect signal any eight examined is approximately 100 picograms. level precision allele size determination observed +/- 0.2 0.5 base pair (intragel) 1.5 pairs (intergel). Consequently, PCR-based primers provides enhanced precision, forensic casework analysis. Moreover, this offers significant advantages routine processing large numbers samples, greatly facilitates expedites generation profile databases enables investigators perform simultaneous survey several loci single individuals and/or