Synthesis and Characterization of Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Polymer Mixtures Produced in High-Density Fed-Batch Cultures of Ralstonia eutropha (Alcaligenes eutrophus)

摘要: Alternate feeding of glucose and propionic acid to phosphate-depleted batch cultures Ralstonia eutropha (reclassified from Alcaligenes eutrophus) produced poly(3-hydroxybutryate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] with thermal properties markedly different random copolymers similar monomer content. These polymers exhibited a single glass transition melting peak that was significantly higher than expected for copolymer. The were found, by solvent fractionation NMR, be mixture poly(3-hydroxybutyrate) [P(3HB)] copolymer, P(3HB-co-3HV). When compared content, the alternate substrate displayed no improvement in mechanical possessed aging characteristics.