Methylmercury Neurotoxicity in Cultures of Human Neurons, Astrocytes, Neuroblastoma Cells

摘要: Abstract Neurotoxic effects of methylmercury were investigated in vitro primary cultures human neurons and astrocytes isolated from fetal brain the neuroblastoma cell line SH-SY5Y. The protection provided by agents with antioxidant properties was tested these to examine oxidative stress mechanism poisoning. After 24 h exposure methylmercury, LC50 values 6.5, 8.1 6.9 μM for neurons, cells, respectively, degree damage increased at longer times. Depletion cellular pool reduced glutathione (GSH) treatment buthionine sulfoximine potentiated cytotoxicity all three types; cells most sensitive. Addition GSH extracellularly blocked neurotoxicity types. major beneficial effect could be attributed its capacity form conjugates which reduces availability organometallic molecules facilitates their efflux. Cysteine protected neurotoxicity, while selenite, Vitamin E catalase produced some minor protective types, particularly neurons. present study showed that neural had differential responses methylmercury: resistant more responsive afforded antioxidants among