A glycerol-3-phosphate dehydrogenase-deficient mutant of Saccharomyces cerevisiae expressing the heterologous XYL1 gene.
作者： G Lidén , M Walfridsson , R Ansell , M Anderlund , L Adler
DOI: 10.1128/AEM.62.10.3894-3896.1996
关键词: Saccharomyces cerevisiae 、 Xylitol 、 Biology 、 Biochemistry 、 Xylose metabolism 、 Xylose 、 Mutant 、 Dehydrogenase 、 Glycerol-3-phosphate dehydrogenase 、 Pichia stipitis
摘要: The gene XYL1, encoding a xylose reductase, from Pichia stipitis was transformed into mutant of Saccharomyces cerevisiae incapable glycerol production because deletion the genes GPD1 and GPD2. strain capable anaerobic glucose conversion in presence added xylose, indicating that reductase reaction can fulfill role glycerol-3-phosphate dehydrogenase as redox sink. specific xylitol rate obtained 0.38 g g-1 h-1.
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