DNA methylation and Dc8-GUS transgene expression in carrot (Daucus carota L.).
作者： Yuanxiang Zhou , J. M. Magill , C. W. Magill , R. J. Newton
DOI: 10.1007/BF00233146
关键词: Somatic embryogenesis 、 Molecular biology 、 Biology 、 Gene expression 、 Daucus carota 、 DNA methylation 、 Plasmid 、 Methylation 、 Gene 、 Transgene
摘要: DNA methylation has been associated with gene activity in differentiating and developing plant tissues. The objective of this study was to determine the involvement expression a transferred into carrot (Daucus carota L.) tissues by particle bombardment. Expression Dc8-GUS construct response treatments 5-azacytidine (S-azaC) vitro methylases investigated histochemical assay GUS activity. 5-azaC treatment increased frequency Dc8-driven both calli somatic embryos. increase occurred either E. coli containing plasmid insert or before expression, treatment, enhanced ABA embryos more prominent latter. Increased digestion 5-azaC-treated EcoRII suggested that demethylation had occurred. In generally resulted lower expression. SssI methylase completely inhibited level correlated extent plasmid.
springer.com
sci-hub.se 参考文章(16)
F. Creusot, G. Acs, J.K. Christman, Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidine and 5-aza-2'-deoxycytidine. Journal of Biological Chemistry. ,vol. 257, pp. 2041- 2048 ,(1982) , 10.1016/S0021-9258(19)68144-5
Reinhard Töpfer, Jozef Schell, Hans-Henning Steinbiss, Versatile cloning vectors for transient gene expression and direct gene transfer in plant cells Nucleic Acids Research. ,vol. 16, pp. 8725- 8725 ,(1988) , 10.1093/NAR/16.17.8725
M. Guiltinan, W. Marcotte, R. Quatrano, A plant leucine zipper protein that recognizes an abscisic acid response element Science. ,vol. 250, pp. 267- 271 ,(1990) , 10.1126/SCIENCE.2145628
Polydefkis Hatzopoulos, Franklin Fong, Z. Renee Sung, Abscisic Acid Regulation of DC8, A Carrot Embryonic Gene Plant Physiology. ,vol. 94, pp. 690- 695 ,(1990) , 10.1104/PP.94.2.690
Gretchen Kirchner, Carla J. Kinslow, Gregory C. Bloom, David Winship Taylor, Nonlethal assay system of β-glucuronidase activity in transgenic tobacco roots Plant Molecular Biology Reporter. ,vol. 11, pp. 320- 325 ,(1993) , 10.1007/BF02905333
Richard I.S. Brettell, Elizabeth S. Dennis, Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern. Molecular Genetics and Genomics. ,vol. 229, pp. 365- 372 ,(1991) , 10.1007/BF00267457
Reinhard Kunze, Peter Starlinger, Drew Schwartz, DNA methylation of the maize transposable element Ac interferes with its transcription Molecular Genetics and Genomics. ,vol. 214, pp. 325- 327 ,(1988) , 10.1007/BF00337730
Gorm Palmgren, Ole Mattson, Finn Thyge Okkels, Treatment of Agrobacterium or leaf disks with 5-azacytidine increases transgene expression in tobacco. Plant Molecular Biology. ,vol. 21, pp. 429- 435 ,(1993) , 10.1007/BF00028801
Manfred Klaas, Manorama C. John, Dring N. Crowell, Richard M. Amasino, Rapid induction of genomic demethylation and T-DNA gene expression in plant cells by 5-azacytosine derivatives. Plant Molecular Biology. ,vol. 12, pp. 413- 423 ,(1989) , 10.1007/BF00017581
B. F. VANYUSHIN, A. N. BELOZERSKY, N. A. KOKURINA, D. X. KADIROVA, 5-Methylcytosine and 6-Methylaminopurine in Bacterial DNA Nature. ,vol. 218, pp. 1066- 1067 ,(1968) , 10.1038/2181066A0