Expression of xenobiotic-metabolizing cytochrome P450 Forms in human full-term placenta
作者： Jukka Hakkola , Markku Pasanen , Janne Hukkanen , Olavi Pelkonen , Jukka Mäenpää
DOI: 10.1016/0006-2952(95)02184-1
关键词: CYP3A7 、 CYP2B6 、 CYP1A2 、 Cytochrome P450 、 Aromatase 、 CYP2A6 、 Gene expression 、 Placenta 、 Endocrinology 、 Biology 、 Internal medicine
摘要: The expression of individual xenobiotic-metabolizing cytochrome P450 (CYP) genes in human placenta was studied at the mRNA level by reverse transcriptase-polymerase chain reaction (RT-PCR). mRNAs CYP1A1, CYP2E1, CYP2F1, CYP3A3/4, CYP3A5, and CYP4B1 were detected RT-PCR, CYP1A2, CYP2A6/7, CYP2B6/7, CYP2C8-19, CYP2D6, CYP3A7 not detected. Several enzyme activity assays immunoblots used to further characterize forms producing detectable transcripts. catalytic activities 7-ethoxycoumarin O-deethylase (ECOD), 7-ethoxyresorufin (EROD) aryl hydrocarbon hydroxylase (AHH) substantially increased response maternal cigarette smoking, paralleled amount CYP1A1 protein. Aromatase slightly lower placentas exposed smoke compared with nonexposed placentas. These data show that several CYP are expressed a low level. significance such low-level is unknown, but it may have local physiological or toxic consequences.
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