Physical mapping of four sites of 5S rDNA sequences and one site of the α-amylase-2 gene in barley (Hordeum vulgare).

摘要: The 5S rDNA sequences have been mapped on four pairs of barley (Hordeum vulgare L.) chromosomes using in situ hybridization and monotelotrisomic lines. are located, genetically physically, the short arm chromosome 1 (7I) long arms 2 (2I) 3 (3I). sequence is also located physically 4 (4I). Only one site 2(2I) has previously reported. characteristic locations make them useful as molecular markers to identify each chromosome. physical position low-copy α-amylase-2 gene was determined hybridization; location this confirmed by reprobing same preparation with probe. results show that there a discrepancy between genetic gene.Key words: mapping, barley, DNA, α-amylase, ...