Induction and morpho-chemical characterization of Stevia rebaudiana colchiploids
作者： D Dhyani , Bikram Singh , Ashok Kumar Yadav , Anjali Sharma , Garima Bhardwaj
DOI:
关键词: Leaf size 、 Ploidy 、 Glycoside 、 Callus 、 Stevia rebaudiana 、 Botany 、 Biology 、 Germplasm 、 Colchicine 、 Stevia
摘要: A study was conducted to produce higher biomass-yielding stevia Stevia rebaudiana (Bertoni) with larger leaf size and high glycoside content. To improve yield and sweet content found in the leaves of plant, polyploidy was induced by treating seeds colchicine. Treatment 0.2% colchicine above (up 0.6%) reduced survival rate young seedlings below 50%. Maximum number polyploids (tetraploids) were obtained 0.6% treatment for 24 hr; mixoploidy observed at 6 hr. It however needs further experimentation more precise information. The nuclear DNA (2C-value diploid Stevia variety Madhuguna) estimated flow-cytometry (FCM) be 2.72pg. Polyploids C7-3-4, C7-3- 5, C9-1-1 C9-4-3 could distinguished from diploid control plants as they had double content, suggesting autotetraploidy. Chromosome count root-tip cells (2n=44) confirmed their tetraploid status compared with (2n=22). autotetraploids significantly increased size, thickness chlorophyll content reduced internode length. Tetraploid genotypes are important germplasm improvement of stevia biomass
csircentral.net
researchgate.net 参考文章(19)
N W Megeji, Virendra Singh, J K Kumar, Paramvir Singh Ahuja, V K Kaul, Introducing Stevia rebaudiana, a natural zero-calorie sweetener. Current Science. ,vol. 88, pp. 801- 804 ,(2005)
N.G Galiano, Estudios cromosomicos en especies argentinas de stevia (compositae) Darwiniana. ,vol. 28, pp. 311- 315 ,(1987)
T Rajasekaran, P Giridhar, GA Ravishankar, Production of steviosides in ex vitro and in vitro grown Stevia rebaudiana Bertoni Journal of the Science of Food and Agriculture. ,vol. 87, pp. 420- 424 ,(2007) , 10.1002/JSFA.2713
Ives Vanstechelman, Tom Eeckhaut, Johan Van Huylenbroeck, Marie-Christine Van Labeke, Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii Euphytica. ,vol. 174, pp. 61- 72 ,(2010) , 10.1007/S10681-010-0133-Z
A. A. Pinheiro, M. T. Pozzobon, C.B. do Valle, M. I. O. Penteado, V. T. C. Carneiro, Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine. Plant Cell Reports. ,vol. 19, pp. 274- 278 ,(2000) , 10.1007/S002990050011
Paul G. Bartels, James L. Hilton, Comparison of trifluralin, oryzalin, pronamide, propham, and colchicine treatments on microtubules Pesticide Biochemistry and Physiology. ,vol. 3, pp. 462- 472 ,(1973) , 10.1016/0048-3575(73)90072-2
Martin Pfosser, Erwin Heberle-Bors, Amelia Amon, Thomas Lelley, Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat–rye addition lines Cytometry. ,vol. 21, pp. 387- 393 ,(1995) , 10.1002/CYTO.990210412
Y. Wan, J. F. Petolino, J. M. Widholm, Efficient production of doubled haploid plants through colchicine treatment of anther-derived maize callus. Theoretical and Applied Genetics. ,vol. 77, pp. 889- 892 ,(1989) , 10.1007/BF00268344
Jaroslav Doležel, Johann Greilhuber, Jan Suda, Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols. ,vol. 2, pp. 2233- 2244 ,(2007) , 10.1038/NPROT.2007.310
J. Loureiro, E. Rodriguez, J. Dolezel, C. Santos, Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species. Annals of Botany. ,vol. 100, pp. 875- 888 ,(2007) , 10.1093/AOB/MCM152