Studies on cyclic AMP levels and phosphodiesterase activity in developing sea urchin eggs: Effects of puromycin, 6-dimethylamino purine and aminophylline
作者： J. Nath , L.I. Rebhun
DOI: 10.1016/0014-4827(73)90583-1
关键词: Intracellular 、 Biochemistry 、 Cyclic adenosine monophosphate 、 Sea urchin 、 Biology 、 Nucleotide 、 Puromycin 、 Phosphodiesterase 、 Purine 、 Aminophylline
摘要: Abstract Cyclic adenosine monophosphate (CAMP) was measured in sea urchin eggs by the binding assay method of Gilman and with a radioimmune procedure. Intracellular concentrations nucleotide unfertilized were about 1.5 × 10−7 M rose to 3 times this value at first cleavage. Aminophylline, known inhibitor phosphodiesterase shown cause an increase intracellular levels CAMP cleavage inhibit activity homogenates both fertilized eggs. Puromycin its purine component, 6-dimethylaminopurine (DMAP), did not order magnitude higher than those which they cell division.
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