LINC01018 and SMIM25 sponged miR-182-5p in endometriosis revealed by the ceRNA network construction.

摘要: The current study intended to explore the interaction of long non-coding RNA (lncRNA), microRNA (miRNA), and messenger (mRNA) under background competitive endogenous (ceRNA) network in endometriosis (EMs). differentially expressed miRNAs (DEmiRs), lncRNA (DELs), genes (DEGs) between EMs ectopic (EC) eutopic (EU) endometrium based on three RNA-sequencing datasets (GSE105765, GSE121406, GSE105764) were identified, which used for construction ceRNA network. Then, DEGs performed with Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway, protein-protein (PPI) analysis. Besides, DEmiRs validated GSE124010. And target DELs verified GSE86534. correlation DEmiRs, DEGs, was explored. Moreover, gene set enrichment analysis (GSEA) applied investigate function DELs. Overall, 1352 595 from GSE105764, along 27 overlapped GSE105765 obtained. Subsequently, a network, including 11 upregulated 16 downregulated 7 13 DELs, 48 46 constructed. GO KEGG pathway showed that this probably associated inflammation-related pathways. Furthermore, hsa-miR-182-5p its (LINC01018 SMIM25) (BNC2, CHL1, HMCN1, PRDM16) successfully validation significantly negatively correlated these DEGs. GSEA implied high expression LINC01018, SMIM25, low would activate pathways EU samples.LINC01018 SMIM25 might sponge upregulate downstream such as CHL1 promote development endometriosis.