Highly sensitive and selective chip-based fluorescent sensor for mercuric ion: development and comparison of turn-on and turn-off systems.

摘要: Miniaturization is currently an important trend in environmental and food monitoring because it holds great promise for on-site detection. We report here two ready-to-use chip-based fluorescent sensors, compatible with microarray technology reagentless, one-step, fast, highly sensitive selective detection of the mercuric ion (Hg2+) turn-on turn-off operation modes. Both modes are based on T–Hg2+–T coordination between neighboring polythymine (T) strands at a high probe density its induced displacement complementary polyadenine strand labeled either fluorophore or quencher, which enables Hg2+, respectively. The sensor slightly more than sensor, their limits 3.6 8.6 nM, respectively, both lower U.S. Environmental Protection Agency limit [Hg2+] drinkable water (10 2 ppb). Compared to sensor...