Staphylococcus aureus DivIB is a peptidoglycan‐binding protein that is required for a morphological checkpoint in cell division

摘要: Bacterial cell division is a fundamental process that requires the coordinated actions of number proteins which form complex macromolecular machine known as divisome. The membrane-spanning DivIB and its orthologue FtsQ are crucial divisome components in Gram-positive Gram-negative bacteria respectively. However, role almost all integral proteins, including DivIB, still remains largely unknown. Here we show extracellular domain able to bind peptidoglycan have mapped binding β subdomain. Conditional mutational studies divIB essential for Staphylococcus aureus growth, while phenotypic analyses following depletion results block completion, but not initiation, septum formation. Localisation suggest only transiently localises site may mark previous sites septation. We propose required molecular checkpoint during ensure correct assembly at midcell prevent hydrolytic growth absence completed septum.