The myosin phosphatase targeting protein (MYPT) family: a regulated mechanism for achieving substrate specificity of the catalytic subunit of protein phosphatase type 1δ.

摘要: Abstract The mammalian MYPT family consists of the products five genes, denoted MYPT1, MYPT2, MBS85, MYPT3 and TIMAP, which function as targeting regulatory subunits to confer substrate specificity subcellular localization on catalytic subunit type 1δ protein serine/threonine phosphatase (PP1cδ). Family members share several conserved domains, including an RVxF motif for PP1c binding ankyrin repeats that mediate protein–protein interactions. MYPT2 MBS85 contain C-terminal leucine zipper domains involved in dimerization interaction, whereas TIMAP are targeted membranes via a prenylation site. All regulated by phosphorylation at multiple sites various kinases; example, Rho-associated kinase phosphorylates resulting inhibition activity Ca2+ sensitization smooth muscle contraction. A great deal is known about myosin light chain phosphatase, terms its role regulation contraction and, lesser extent, non-muscle motile processes. appears be key cardiac skeletal muscles. most closely resembles but little physiological function. Little also MYPT3, although it likely target thereby achieve substrates actin cytoskeleton. cAMP-dependent kinase. PP1cδ plasma membrane endothelial cells where serves dephosphorylate proteins cytoskeleton control barrier With such wide range targets, have been implicated diverse pathological events, hypertension, Parkinson’s disease cancer.