Evidence from a genetic fate-mapping study that stem cells refresh adult mammalian cardiomyocytes after injury

摘要: An emerging concept is that the mammalian myocardium has potential to regenerate, but regeneration might be too inefficient repair extensive myocardial injury typical of human disease. However, degree which stem cells or precursor contribute renewal adult cardiomyocytes remains controversial. Here we report evidence replacement after do not significantly cardiomyocyte during normal aging. We generated double-transgenic mice track fate in a 'pulse-chase' fashion: 4-OH-tamoxifen pulse, green fluorescent protein (GFP) expression was induced only cardiomyocytes, with 82.7% expressing GFP. During aging up one year, percentage GFP+ remained unchanged, indicating did refresh uninjured at significant rate this period time. By contrast, infarction pressure overload, decreased from 82.8% heart tissue sham-treated 67.5% areas bordering infarction, 76.6% away and 75.7% hearts subjected had refreshed cardiomyocytes.