Multicolor Imaging with Fluorescent Proteins in Mice

作者: Robert M. Hoffman

DOI: 10.1007/978-1-4419-1260-2_11

关键词: Tumor Cell MobilityCell biologyChemistryCell typeIn vivoMetastasisCancerCancer cellAngiogenesisFluorescence

摘要: The high extinction coefficients, quantum yields, and unique spectral properties of fluorescent proteins are optimal for imaging in live animals real time. important aspects cancer living animals, including tumor cell mobility, invasion, metastasis, angiogenesis, can be imaged. Fluorescent enable whole-body tumors on internal organs. Proteins which spectrally distinct have allowed the color coding cells growing vivo with distinction different types, host from tumor, single-cell resolution. Individual multicolored to study nuclear–cytoplasmic dynamics animal.

参考文章(112)
Dai Fukumura, Fan Yuan, Rakesh K. Jain, Wayne L. Monsky, Yi Chen, Effect of host microenvironment on the microcirculation of human colon adenocarcinoma. American Journal of Pathology. ,vol. 151, pp. 679- 688 ,(1997)
Jörg W. Sturm, Michael A. Keese, Beate Petruch, Roderich G. Bönninghoff, Hongyue Zhang, Norbert Gretz, Mattias Hafner, Stefan Post, Robert S. Mc Cuskey, Enhanced green fluorescent protein-transfection of murine colon carcinoma cells: key for early tumor detection and quantification. Clinical & Experimental Metastasis. ,vol. 20, pp. 395- 405 ,(2003) , 10.1023/A:1025470312074
Lothar Hennighausen, John S. Condeelis, Jeffrey E. Segall, Jeffrey W. Pollard, Elaine Y. Lin, Weigang Wang, Yarong Wang, Jun ichi Miyazaki, Fayyaz Ahmed, Jeffrey Wyckoff, Joan Jones, GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice. Cancer Research. ,vol. 62, pp. 7166- 7169 ,(2002)
S Zolotukhin, M Potter, W W Hauswirth, J Guy, N Muzyczka, A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells. Journal of Virology. ,vol. 70, pp. 4646- 4654 ,(1996) , 10.1128/JVI.70.7.4646-4654.1996
John S. Condeelis, Jeffrey E. Segall, Johanna Rivera, Joan G. Jones, Herbert Lee, Kim L. Farina, Jeffrey B. Wyckoff, Cell Motility of Tumor Cells Visualized in Living Intact Primary Tumors Using Green Fluorescent Protein Cancer Research. ,vol. 58, pp. 2528- 2532 ,(1998)
D.A. Tyas, T. Pratt, T.I. Simpson, J.O. Mason, D.J. Price, Identifying GFP-transgenic animals by flashlight. BioTechniques. ,vol. 34, pp. 474- 476 ,(2003) , 10.2144/03343BM04
A.B. Al-Mehdi, K. Tozawa, A.B. Fisher, L. Shientag, A. Lee, R.J. Muschel, Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis. Nature Medicine. ,vol. 6, pp. 100- 102 ,(2000) , 10.1038/71429
Yuichiro Doki, Morito Monden, Yoshiyuki Fujiwara, Hitoshi Shiozaki, Katsuhiko Kaneko, Yosuke Akiyama, Toshimasa Tsujinaka, Tomoki Yamano, Masahiko Yano, Masatoshi Inoue, Yasushi Kaneda, Masaaki Taniguchi, Hirofumi Miki, Detection of peritoneal micrometastases of gastric carcinoma with green fluorescent protein and carcinoembryonic antigen promoter. Cancer Research. ,vol. 61, pp. 5570- 5574 ,(2001)
Ming-Shyan Huang, Tzu-Jou Wang, Chung-Ling Liang, Huey-Mei Huang, I-Chi Yang, Yi-Hua Jan, Michael Hsiao, Establishment of fluorescent lung carcinoma metastasis model and its real-time microscopic detection in SCID mice. Clinical & Experimental Metastasis. ,vol. 19, pp. 359- 369 ,(2002) , 10.1023/A:1015562532564
John S. Condeelis, Jeffrey E. Segall, Jeffrey B. Wyckoff, Joan G. Jones, A Critical Step in Metastasis: In Vivo Analysis of Intravasation at the Primary Tumor Cancer Research. ,vol. 60, pp. 2504- 2511 ,(2000)