Formation of a protein monomolecular layer by a combined technique of LB and SA methods.
作者： Jong Bum Lee , Dae-Jin Kim , Jeong-Woo Choi , Kee-Kahb Koo
DOI: 10.1016/J.COLSURFB.2004.12.003
关键词: Langmuir–Blodgett film 、 Substrate (chemistry) 、 Cytochrome c 、 Layer (electronics) 、 Chemisorption 、 Stereochemistry 、 Crystallography 、 Scanning tunneling microscope 、 Monolayer 、 Chemistry 、 Protein aggregation
摘要: This paper describes a technique developed for the formation of self-assembled protein monomolecular layer. The main idea is direct transfer molecules consisting Langmuir-Blodgett (LB) film onto surface another chemically modified metal substrate and induction spontaneous monolayer (SAM) by chemisorption without aggregates. In present experiments, cytochrome c (cyt c) SAM on gold was prepared incubating EDC/MUA-modified overlaid with cyt LB in phosphate buffer solution. Scanning tunneling microscopy (STM) image shows that size clusters most part approximately 5 nm, indicating rectifying property confirmed to remain an asymmetric I-V curve applied bias from -1 V 1 V.
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