Two Phages, phiIPLA-RODI and phiIPLA-C1C, Lyse Mono- and Dual-Species Staphylococcal Biofilms

摘要: Phage therapy is a promising option for fighting against staphylococcal infections. Two lytic phages, vB_SauM_phiIPLA-RODI (phiIPLA-RODI) and vB_SepM_phiIPLA-C1C (phiIPLA-C1C), belonging to the Myoviridae family exhibiting wide host ranges, were characterized in this study. The complete genome sequences comprised 142,348 bp 140,961 contained 213 203 open reading frames, respectively. gene organization was typical of Spounavirinae members, with long direct terminal repeats (LTRs), genes grouped into modules not clearly separated from each other, several group I introns. In addition, four encoding tRNAs identified phiIPLA-RODI. Comparative DNA sequence analysis showed high similarities two GH15 676Z, Twort-like virus genus (nucleotide identities >84%); phiIPLA-C1C, similarity phage phiIBB-SEP1 observed (identity 80%). Challenge assays phages phiIPLA-RODI phiIPLA-C1C planktonic cells confirmed their ability, as they able remove 5 log units 8 h. Exposure biofilms reduced amount adhered bacteria about 2 both monospecies dual-species biofilms, but turned out be effective mixture phages. Moreover, frequencies bacteriophage-insensitive mutants (BIMs) Staphylococcus aureus S. epidermidis resistance low, at 4.05 × 10−7 ± 2.34 10−9 1.1 2.08 10−9, Overall, generally fitness absence BIMs, which restored phage-sensitive phenotype few generations. These results confirm that bacteriophages can efficient biofilm-disrupting agents, supporting potential antimicrobials