Acute Effects of Estradiol and Follicle-Stimulating Hormone on Specific Binding of Human [125I]Iodo-Follicle-Stimulating Hormone to Rat Ovarian Granulosa Cells in vivo and in vitro*

摘要: Twenty-four-day-old hypophysectomized and intact immature female rats were used to determine the time-dependent effects of estradiol on FSH stimulation receptor in granulosa cells. In rats, a single 2 μg injection human (h) (LER-1577) given alone, concomitantly with, or 6 h after 1.5 mg caused 50% increase available content cells by 24 h. The same dose hFSH 12 300% To establish accuracy these vitro FSH-binding assays, it was imperative if administered vivo occupied binding sites thereby masked increases total occurring within first Therefore, 20 × 106 cpm 125I-labeled hFSH, equivalent 250 ng LER-1577, sc estradiolpiimed 1 an saline hFSH. amount labeled hormone bound 30,000...