Studies on the biosynthesis of collagen. I. The growth of fowl osteoblasts and the formation of collagen in tissue culture.

摘要: 1. A tissue culture method was devised in which suspensions of osteoblasts, obtained directly from frontal bones fowl embryos, were grown a fluid, fibrin-free medium. 2. Maximum growth the tissue, as measured by dry weight, with formation collagen protein, based on estimation hydroxyproline, periods up to 6 days. 3. Appreciable amounts protein-bound hydroxyproline formed during first 24 hour period, but electron microscopy portions same cultures failed demonstrate presence any typical fibrils. 4. The subsequent many characteristic fibrils not associated significant rise mean content tissue. 5. cytoplasmic granules osteoblasts stained intensely P.A.S. technique when being formed. 6. It is suggested that collagen-forming cells synthesise and secrete hydroxyproline-rich precursor protein or large peptide nature, subsequently becomes transformed into