Immunogenicity and protective efficacy of recombinant Modified Vaccinia virus Ankara candidate vaccines delivering West Nile virus envelope antigens
作者： Asisa Volz , Stephanie Lim , Martina Kaserer , Anna Lülf , Lisa Marr
DOI: 10.1016/J.VACCINE.2016.02.042
关键词: Viral vector 、 Virus 、 Virology 、 Immunogenicity 、 Antigen 、 Viral Vaccine 、 Flavivirus 、 Biology 、 West Nile Virus Vaccines 、 Vaccinia
摘要: West Nile virus (WNV) cycles between insects and wild birds, is transmitted via mosquito vectors to horses humans, potentially causing severe neuroinvasive disease. Modified Vaccinia Ankara (MVA) an advanced viral vector for developing new recombinant vaccines against infectious diseases cancer. Here, we generated evaluated MVA candidate that deliver WNV envelope (E) antigens fulfil all the requirements proceed clinical testing in humans. Infections of human equine cell cultures with demonstrated efficient synthesis secretion proteins mammalian cells non-permissive replication. Prime-boost immunizations BALB/c mice readily induced circulating serum antibodies binding E protein neutralizing tissue culture infections. Vaccinations HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout elicited E-specific CD8+ T responses. Moreover, MVA-WNV protected C57BL/6 lineage 1 2 infection heterologous antibodies. Thus, further studies are warranted evaluate these other preclinical models use them as vaccine
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