Intracellular cleavage and ligation of hepatitis delta virus genomic RNA: regulation of ribozyme activity by cis-acting sequences and host factors.

摘要: During replication, a ribozyme within the genomic RNA of hepatitis delta virus cleaves multimeric precursors to release unit-length linear intermediate. Intramolecular ligation this intermediate produces circular RNA. Although one copy is reconstituted by such ligation, it does not subsequently cleave and destroy conformation. We have identified cis-acting attenuator sequences that prevent self-cleavage product base pairing with inactivating ribozyme. Furthermore, we shown during initial processing precursor RNA, host-specific factors activate preventing its association sequences. Thus, demonstrate novel switching mechanism regulates activity inside cell.