Cell Distribution of Stress Fibres in Response to the Geometry of the Adhesive Environment

作者: Manuel Théry , Anne Pépin , Emilie Dressaire , Yong Chen , Michel Bornens

DOI: 10.1002/CM.20126

关键词: CytoskeletonStress (mechanics)AdhesiveBiologyActinTension (geology)GeometryCell typeTraction (engineering)Focal adhesion

摘要: Cells display a large variety of shapes when plated in classical culture conditions despite their belonging to common cell type. These are transitory, since cells permanently disassemble and reassemble cytoskeleton while moving. Adhesive micropatterns commonly used confine shape within given geometry. In addition the micropattern can be designed so as impose spread upon adhesive nonadhesive areas. Modulation pattern geometry allows analysis mechanisms governing determination response external conditions. this study, we show that acquisition follows two stages where initially forms contact with micropattern. Here, most distal contacts made by define apices shape. Then secondly, borders link move minimise distance between apices. these borders, absence an underlying substrate is overcome stress fibres forming apices, which turn marked accumulation focal adhesions. By inhibiting myosin function, on zones become more concave, suggesting work against membrane tension border. Moreover, suggested traction forces unevenly distributed stationary, nonmigrating, cells. comparing one, two, or three nonadherent it was reasoned fibre strength inversely proportional number. We conclude area generate same total sum tractional but differently spaced depending spatial distribution its adherence contacts. Cell Motil. Cytoskeleton 63:341–355, 2006. ' 2006 Wiley-Liss, Inc.

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