Simple Procedure for Determining Octanol–Aqueous Partition, Distribution, and Ionization Coefficients by Reversed-Phase High-Pressure Liquid Chromatography

摘要: The described simple, accurate, and precise reversed-phase high-pressure liquid chromatographic procedure is in excellent agreement with 1-octanol shake-flask partition or distribution coefficients over a 3.5 log range. A chemically bonded octadecylsilane support persilated coated 1-octanol. With 1-octanol-saturated buffers as mobile phases, stable baseline (compared to adsorbed on silica) obtained rapidly, the relative retention times are highly correlated unit slope from classical procedures. Only relatively basic, unhindered pyridines deviate, probably because of binding residual silinol sites. In addition, if apparent pKa pKab an ionizable compound lies within pH operating range column support, usually can be determined simultaneously P by measuring coefficient at several values. gives rapid results, requires little material, tolerate impurities.