Mechanism of antiproliferative action of a new d-secoestrone-triazole derivative in cervical cancer cells and its effect on cancer cell motility.

摘要: Abstract Cervical cancer is the fourth most frequently diagnosed tumor and leading cause of death in females worldwide. predominantly related with human papilloma virus (HPV) infection, oncogenic types being HPV-18 -16. Our previous studies demonstrated that some d -secoestrone derivatives exert pronounced antiproliferative activity. The aim current investigation was to characterize mechanism action -secoestrone-triazole (D-SET) on three cervical cell lines different pathological backgrounds. growth-inhibitory effects D-SET were determined by a standard MTT assay. We have found exerts effect HPV 18-positive HeLa HPV-negative C-33 A cells, but it has no substantial inhibitory activity 16-positive SiHa or intact fibroblast MRC-5 lines. After 24 h incubation, cells showed morphological biochemical signs apoptosis fluorescent double staining, flow cytometry caspase-3 Besides elevation ratio subG1 phase, cytometric analysis revealed cycle arrest at G2/M both To distinguish population immunocytochemical performed cells. results show significantly increases phosphorylated histone H3, indicating accumulation M phase. Additionally, increased maximum rate microtube formation measured an vitro tubulin polymerization its direct activity, antimigratory property been investigated. demonstrate inhibits migration invasion after incubation. These suggests potent agent against 16+ lines, efficacious motility-inhibiting Accordingly can be regarded as potential drug candidate promising new among steroids, potentially allowing for design novel anticancer agents.