Urokinase activity in corneal fibroblasts may be modulated by DNA damage and secreted proteins.

摘要: Proteases like urokinase-type plasminogen activator (uPA) play an important role in tumor invasion. Cells derived from ultraviolet radiation (UVR)-induced corneal sarcomas of Monodelphis domestica produce relatively high levels uPA compared to the untransformed keratocytes suggesting a mechanism for their invasiveness. Because UVR is known stimulate production many cell types, exposure may further increase expression cells, thus enhancing ability infiltrate. We investigated control basal and induction by transformed Monodelphis. These studies took advantage fact that possesses active photolyase can be stimulated remove UVR-induced pyrimidine dimers long-wavelength visible photoreactivating light (PRL). Our showed significant occurred response 200 J/m2 UVR. This was partially blocked treatment with PRL, indicating DNA damage, dimer particular, played induction. In cultured fibroblasts, heparin-binding protein inhibitor, suramin, reduced levels, proliferation. Basic fibroblast growth factor, factor UVR-inducible mesenchymal proliferation; however, anti-bFGF antibodies did not significantly decrease proliferation or production. findings suggested secretion were modulated factors these also have mediated effect on levels.