Assay of the retinoid isomerase system of the eye.

作者: Paul S. Bernstein , Robert R. Rando

DOI: 10.1016/0076-6879(90)89327-E

关键词: BiochemistryIsomeraseRetinoid isomeraseVisual phototransductionMetabolismMechanism of actionEnzymeRetinoic acidChemistryRetinal isomerase

摘要: Publisher Summary The discovery and characterization of the retinoid isomerase have significant implications. It is an enzyme that completes a fundamental biochemical cycle apparently common to all vertebrate visual systems. also first known whose physiological task drive enzymatic conversion trans double bond higher energy cis configuration. This chapter describes methods used assay this important enzyme. clear isomerase, system bleaching dark adaptation, will role in physiology pathophysiology vision, as well providing insights into study enzymes general. Future purification novel class should clarify its relationship other enzymes, regulation, mechanism action.

参考文章(14)
P. Gouras, M. T. Flood, W. S. Blaner, C. D. D. Bridges, R. A. Alvarez, Vitamin A utilization in human retinal pigment epithelial cells in vitro. Investigative Ophthalmology & Visual Science. ,vol. 24, pp. 1227- 1235 ,(1983)
P S Bernstein, W C Law, R R Rando, Biochemical characterization of the retinoid isomerase system of the eye. Journal of Biological Chemistry. ,vol. 262, pp. 16848- 16857 ,(1987) , 10.1016/S0021-9258(18)45461-0
P. Deigner, W. Law, F. Canada, R. Rando, Membranes as the energy source in the endergonic transformation of vitamin A to 11-cis-retinol. Science. ,vol. 244, pp. 968- 971 ,(1989) , 10.1126/SCIENCE.2727688
Robert R. Rando, Andrew Chang, Studies on the catalyzed interconversions of vitamin A derivatives Journal of the American Chemical Society. ,vol. 105, pp. 2879- 2882 ,(1983) , 10.1021/JA00347A058
S.-L. Fong, C.D.B. Bridges, R.A. Alvarez, Utilization of exogenous retinol by frog pigment epithelium Vision Research. ,vol. 23, pp. 47- 52 ,(1983) , 10.1016/0042-6989(83)90040-8
Paul S. Bernstein, John R. Lichtman, Robert R. Rando, Nonstereospecific biosynthesis of 11-cis-retinal in the eye. Biochemistry. ,vol. 24, pp. 487- 492 ,(1985) , 10.1021/BI00323A036
Paul S. Bernstein, Robert R. Rando, In vivo isomerization of all-trans- to 11-cis-retinoids in the eye occurs at the alcohol oxidation-state Biochemistry. ,vol. 25, pp. 6473- 6478 ,(1986) , 10.1021/BI00369A020