Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting
作者： G J Churchyard , V N Chihota , K Fielding , A D Grant , D Muirhead
DOI:
关键词: Tuberculosis 、 Positive culture 、 Sputum 、 Tuberculosis diagnosis 、 Mycobacterium 、 Mycobacterium tuberculosis 、 Resource constrained 、 National health 、 Surgery 、 Veterinary medicine 、 Medicine
摘要: SETTING: National Health Laboratory Services tuberculosis (TB) laboratory, South Africa. OBJECTIVES: To compare Mycobacterium Growth Indicator Tube (MGIT) with Lowenstein-Jensen (U) medium regard to yield, time positive culture and contamination, assess MGIT cost-effectiveness. DESIGN: Sputum from gold miners was cultured on U. We estimated cost per culture, and, for smear-negative samples, incremental additional M. gained using a decision-tree model. RESULTS: Among 1267 specimens, vs. LJ gave higher yield of mycobacteria (29.7% 22.8%), contamination (16.7% 9.3%) shorter (median 14 25 days specimens). samples that were culture-positive but negative/contaminated LJ, 77.3% non-tuberculous (NTM). Cost MGIT+LJ respectively US$12.35, US$16.62 US$19.29. The identified by standard biochemical tests microscopic cording US$504.08 US$328.10 or US$160.80 US$109.07 U alone. CONCLUSION: gives faster results at relatively high cost. proportion NTM underscores the need rapid speciation tests. Minimising contaminated cultures is key
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