Evaluation of the effects of bioaugmentation and biostimulation on natural attenuation and biodegradation pathways of chlorinated compounds in a tidal wetland

摘要: Title of Document: EVALUATION OF THE EFFECTS BIOAUGMENTATION AND BIOSTIMULATION ON NATURAL ATTENUATION BIODEGRADATION PATHWAYS CHLORINATED COMPOUNDS IN A TIDAL WETLAND Emily N. Devillier, Master Science, 2006 Directed By: Assistant Professor, Jennifer G. Becker, Department Environmental Science and Technology (formerly Biological Resources Engineering) The usefulness bioaugmentation biostimulation in enhancing the natural attenuation chlorinated ethanes, ethenes, methanes at a seep site Aberdeen Proving Ground (APG), MD was tested. biodegradation (1) mixture 1,1,2,2-tetrachloroethane (TeCA), tetrachloroethene (PCE), carbon tetrachloride (CT), or (2) TeCA alone compared sediment groundwater microcosms amended with substrates alone, electron donor, substrates, donor TeCA-degrading enrichment culture. third experiment evaluated H2 thresholds determining importance co-metabolic metabolic processes substrates. Biostimulation did not significantly affect substrate removal. Biodegradation enhanced by addition donors coupled bioaugmentation. However, presence other contaminants, especially appeared to inhibit biodegradation, even exogenous prove useful transformation CT, PCE, TeCA; however, evaluating each compound individually provided insight regards pathways followed effects on degradation rates. Overall, results provide evidence that when assessing contaminated solvents, it is necessary examine contaminant as mixture, order develop successful remediation plan. Evaluation Effects Bioaugmentation Natural Attenuation Pathways Chlorinated Compounds Tidal Wetland