Immobilized GDEst-95, GDEst-lip and GD-95RM lipolytic enzymes for continuous flow hydrolysis and transesterification reactions.

摘要: Abstract In this study lipolytic biocatalysts GD-95RM, GDEst-95 and GDEst-lip were immobilized by encapsulation in calcium alginate beads. All three demonstrated significantly increased thermal stability at 60–70 °C temperatures the activity of GD-95RM lipase 50% 70–80 °C following immobilization. Moreover, encapsulated esterase retained higher than after 3 months incubation with butanol (25%) ethanol (50%); enzyme possessed 2 months treatment methanol (25%); displayed that two-week (50%). enzymes long-term storage capability (>50% activity) least until 4 °C. It was also detected can perform flow hydrolysis both avocado oil p-NP dodecanoate prototype packed-bed column reactor. The analysis continuous transesterification or sunflower as substrates confirmed is a useful approach to produce fatty acid alkyl esters.