A new PCR assay for reliable molecular sexing of endangered Tasmanian devils ( Sarcophilus harrisii ) from non-invasive genetic samples
作者： Shelly Lachish , Abraham Passmore , Menna Jones
DOI: 10.1007/S12686-010-9341-Z
关键词: Testis determining factor 、 Multiplex polymerase chain reaction 、 Biology 、 Genetics 、 Microsatellite 、 Threatened species 、 Evolutionary biology 、 Tasmanian devil 、 Sexing 、 Sarcophilus 、 Endangered species
摘要: Sex identification is a critical component of threatened species monitoring programs. For cryptic, rare and endangered that have low detection probabilities, efficient can be achieved by analysing non-invasively collected DNA with molecular genetic techniques. In this study, we report multiplex PCR-based sexing assay for the Tasmanian devil (Sarcophilus harrisii). The uses new species-specific primer set amplifies fragment SRY gene an autosomal microsatellite marker as internal positive control. We show provides highly accurate robust sex (99% accuracy [95%CI 96.5–0.99.8]) where could assigned from tissue, hair faeces. This simple, yet reliable important step towards effective management devils.
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springer.com参考文章(17)
G.E. Truett, P. Heeger, R.L. Mynatt, A.A. Truett, J.A. Walker, M.L. Warman, Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT) BioTechniques. ,vol. 29, pp. 52- 54 ,(2000) , 10.2144/00291BM09
Jorge Ortega, Mar�a Del Rosario Franco, Brice A. Adams, Katherine Ralls, Jesus E. Maldonado, A reliable, non-invasive method for sex determination in the endangered San Joaquin kit fox ( Vulpes macrotis mutica ) and other canids Conservation Genetics. ,vol. 5, pp. 715- 718 ,(2004) , 10.1007/S10592-003-1862-5
Maxine P. Piggott, Andrea C. Taylor, Extensive evaluation of faecal preservation and DNA extraction methods in Australian native and introduced species Australian Journal of Zoology. ,vol. 51, pp. 341- 355 ,(2003) , 10.1071/ZO03012
C.E. Hawkins, C. Baars, H. Hesterman, G.J. Hocking, M.E. Jones, B. Lazenby, D. Mann, N. Mooney, D. Pemberton, S. Pyecroft, M. Restani, J. Wiersma, Emerging disease and population decline of an island endemic, the Tasmanian devil Sarcophilus harrisii Biological Conservation. ,vol. 131, pp. 307- 324 ,(2006) , 10.1016/J.BIOCON.2006.04.010
R. GRIFFITHS, B. TIWARI, Primers for the differential amplification of the sex‐determining region Y gene in a range of mammal species Molecular Ecology. ,vol. 2, pp. 405- 406 ,(1993) , 10.1111/J.1365-294X.1993.TB00034.X
Bruce C. Robertson, Neil J. Gemmell, PCR-based sexing in conservation biology: Wrong answers from an accurate methodology? Conservation Genetics. ,vol. 7, pp. 267- 271 ,(2006) , 10.1007/S10592-005-9105-6
Menna E. Jones, David Paetkau, Eli Geffen, Craig Moritz, Microsatellites for the Tasmanian devil ( Sarcophilus laniarius ) Molecular Ecology Notes. ,vol. 3, pp. 277- 279 ,(2003) , 10.1046/J.1471-8286.2003.00425.X
Hamish McCallum, Menna Jones, Clare Hawkins, Rodrigo Hamede, Shelly Lachish, David L. Sinn, Nick Beeton, Billie Lazenby, Transmission dynamics of Tasmanian devil facial tumor disease may lead to disease-induced extinction. Ecology. ,vol. 90, pp. 3379- 3392 ,(2009) , 10.1890/08-1763.1
Richard M. Engeman, Indexing principles and a widely applicable paradigm for indexing animal populations Wildlife Research. ,vol. 32, pp. 203- 210 ,(2005) , 10.1071/WR03120
Melanie A. Murphy, Lisette P. Waits, Katherine C. Kendall, The influence of diet on faecal DNA amplification and sex identification in brown bears (Ursus arctos). Molecular Ecology. ,vol. 12, pp. 2261- 2265 ,(2003) , 10.1046/J.1365-294X.2003.01863.X