Chemokine Receptor Expression and Function in CD4+T Lymphocytes with Regulatory Activity

摘要: We have investigated the chemokine receptor expression and migratory behavior of a new subset nickel-specific skin-homing regulatory CD4 + T cells (Th IL-10 ) releasing high levels IL-10, low IFN-γ, undetectable IL-4. These inhibit in IL-10-dependent manner capacity dendritic to activate Tc1 Th1 lymphocytes. RNase protection assay FACS analysis revealed vast repertoire receptors on resting Th , including Th1-associated CXCR3 CCR5, Th2-associated CCR3, CCR4, CCR8, latter at higher compared with Th2 cells. The most active chemokines for terms calcium mobilization vitro migration, were order potency: CCL2 (monocyte chemoattractant protein-1, CCR2 ligand), CCL4 (macrophage-inflammatory protein-1β, CCR5 CCL3 protein-1α, CCR1/5 CCL17 (thymus activation-regulated chemokine, CCR4 CCL1 (I-309, CCR8 CXCL12 (stromal-derived factor-1, CXCR4), CCL11 (eotaxin, CCR3 ligand). Consistent down-regulation, activated exhibited reduced or absent response chemokines, but retained significant I-309, monocyte thymus chemokine. which was ineffective lymphocytes, attracted more efficiently than I-309 mRNAs not detected unaffected skin up-regulated site nickel-allergic reaction, an earlier kinetics Results indicate that lymphocytes coexpress functional Th1- receptors, CCR8/I-309-driven recruitment both may be critically involved regulation Th1-mediated allergic disorders.