作者: Yoshinori Harada , Tamotsu Shimada , Jiro Tokita , Hideki Kanbara , Kenichi Watabe
DOI:
关键词: Analytical chemistry 、 Nucleic acid 、 Photomultiplier 、 Fluorescence 、 Scintillator 、 Electrophoresis 、 Glass tube 、 Materials science
摘要: PURPOSE:To obtain a nucleic acid segment detection apparatus capable of detecting all beta-rays from segment, by providing ring shaped scintillator surrounding cylindrical electrophoretic separation gel. CONSTITUTION:A columnar shape gel 10 comprises, for example, composition containing acrylamide in concn. 8-12% and glass tube 14 with diameter 0.5-5mm thickness about 2mm is proper. A has high sensitivity to absorbs max. energy 1.7MeV and, plastic 10mm The molded the present an upper cell 1 at one end thereof other contacted lower 1'. When voltage applied, 12 electrophoretically migrated negative pole positive pole. are converted fluorescence 11 can be detected photomultiplier 13 pulse counter circuit 20.