Blood transcriptional and microRNA responses to short-term exposure to disinfection by-products in a swimming pool

摘要: Abstract Background Swimming in a chlorinated pool results high exposure levels to disinfection by-products (DBPs), which have been associated with an increased risk of bladder cancer. Objectives By studying molecular responses at the blood transcriptome level we examined biological processes these compounds. Methods Whole-genome gene expression and microRNA analysis was performed on samples collected from 43 volunteers before 2 h after 40 min swimming indoor (PISCINAII study). Exposure THMs measured exhaled breath. Heart rate kcal expenditure were as proxies for physical activity. Associations between assessed using multivariate normal models (MVN), correcting age, body mass index sex. A Bonferroni threshold 5% applied. Results MVN-models individual exposures identified 1778 genes 23 microRNAs that significantly least one DBP. Due co-linearity it not possible statistically disentangle DBP those related However, eliminating previously reported transcripts activity large number hits remained exposure. Among those, 9 linked 31 colon Concordant microRNA/mRNA expressions association hsa-mir-22-3p hsa-miR-146a-5p their targets RCOR1 TLR4, both cancer Conclusions Short-term low DBPs shows genomics may be indicative risk.