Fluorescence Spectroscopic Analysis of the Interaction of Papain with Ionic Liquids
作者： Yunchang Fan , Jinlong Yan , Sheli Zhang , Junhai Li , Dong Chen
DOI: 10.1007/S12010-012-9801-X
关键词: Quenching (fluorescence) 、 C4mim 、 Physical chemistry 、 Gibbs free energy 、 Chemistry 、 Hydrogen bond 、 van der Waals force 、 Papain 、 Fluorescence spectroscopy 、 Ionic liquid 、 Organic chemistry
摘要: The interaction between papain and two typical ionic liquids (ILs), 1-octyl-3-methylimidazolium chloride ([C8mim]Cl) 1-butyl-3-methylimidazolium ([C4mim]Cl), was investigated by using fluorescence spectroscopy technique at a pH value of 7.4. results suggested that ILs could quench the intrinsic probably via static quenching mechanism. binding constants were determined employing method. They very small compared with volatile organic solvents, indicating only weak existed. Gibbs free energy change (∆G), enthalpy (∆H), entropy (∆S) during estimated. Negative values these parameters indicated spontaneous process, also implying hydrogen bonding van der Waals forces played important roles in processes.
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