Cell adhesion on supported lipid bilayers functionalized with RGD peptides monitored by using a quartz crystal microbalance with dissipation
作者： Xiaohua Zhu , Zhao Wang , Ansha Zhao , Nan Huang , Huadong Chen
DOI: 10.1016/J.COLSURFB.2014.01.032
关键词: Vesicle fusion 、 Quartz crystal microbalance 、 Adhesion 、 Membrane 、 Lipid bilayer 、 Nanotechnology 、 Biophysics 、 Fluorescence microscope 、 Chemistry 、 Cell adhesion 、 Vesicle 、 Biotechnology 、 Physical and Theoretical Chemistry 、 Colloid and Surface Chemistry 、 Surfaces and Interfaces 、 General Medicine
摘要: Abstract Supported lipid bilayer (SLB) is one of the most widely used structures to mimic cell membranes. To study cell–cell, cell–matrix and cell–material interactions, supported bilayers (SLBs) functionalized with RGD peptides (SLBs–RGD) were prepared by vesicle fusion on a SiO 2 quartz crystal, subsequently bone mesenchymal stem cells (BMSCs) adhesion was analyzed. A crystal microbalance dissipation (QCM-D) utilized detect dynamic adsorption behavior vesicles BMSCs in real time. Observations obtained QCM-D signals are confirmed conducting fluorescence microscopy. measurements showed SLB formation starts at critical concentration vesicles. More adhered SLBs–RGD than SLBs. With presence SLBs, SLBs surfaces had rounded morphology, will take long time rearrange their cytoskeleton, which led incomplete spreading compared . Differences density properties different substrates could be traced versus frequency (Δ D /Δ f ) plots. These results indicate that in/on provide anchorage sites for more adhesion. demonstrated useful tool evaluating interactions between various biological non-biological systems situ real-time.
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