In vitro studies of antifreeze glycoprotein (AFGP) and a C-linked AFGP analogue.

摘要: Antifreeze glycoproteins (AFGPs) are a subclass of biological antifreezes found in deep sea Teleost fish. These compounds have the ability to depress freezing point organism such that it can survive subzero temperatures encountered its environment. This physical property is very attractive for cryopreservation cells, tissues, and organs. Recently, our laboratory has designed synthesized functional carbon-linked (C-linked) AFGP analogue (1) demonstrates tremendous promise as novel cryoprotectant. Herein we describe vitro effects interactions C-linked 1 native 8. Our studies reveal 8 cytotoxic human embryonic liver kidney cells at concentrations higher than 2 0.63 mg/mL, respectively, whereas lower not toxic. The mechanism this cytotoxicity consistent with apoptosis because caspase-3/7 levels significantly elevated cell cultures treated In contrast, displayed no even high concentrations, notably, activities were suppressed well below background lines 1. Although results from these limit applications AFGP, they illustrate benefits developing analogues various medical, commercial industrial applications.