Quantification of Anti-Aggregation Activity of Chaperones: A Test-System Based on Dithiothreitol-Induced Aggregation of Bovine Serum Albumin

摘要: The methodology for quantification of the anti-aggregation activity protein and chemical chaperones has been elaborated. applicability this was demonstrated using a test-system based on dithiothreitol-induced aggregation bovine serum albumin at 45°C as an example. Methods calculating initial rate (vagg) have discussed. comparison dependences vagg concentrations intact cross-linked α-crystallin allowed us to make conclusion that non-linear character dependence concentration due dynamic mobility quaternary structure polydispersity α-crystallin–target complexes. To characterize (arginine, arginine ethyl ester, amide proline), semi-saturation [L]0.5 used. Among studied, ester reveal highest ([L]0.5 = 53 58 mM, respectively).