Host-cell interaction of animal viruses

摘要: Abstract Destruction of mammalian cell reproductive capacity by virus was quantitatively measured attaching Newcastle disease (NDV) to monodisperse HeLa cells up a multiplicity 5 and scoring the fraction survivors as colony counts after 8–10 days' incubation. Single-cell survival experiments in this range attached demonstrate that single particle NDV is sufficient kill cell. Microscopic examination individual cells, 95% which have received killing NDV, reveal no significant difference from uninfected attach, spread on glass 5–15 hours seeding. However, such infected do not undergo even one division. plaque-forming-particle (PFP) hemagglutinating-particle (HAP) curves for ultraviolet radiation heating 55° are presented. Exposure specific antiserum, 55°, or results loss its ability host cells. This technique permits titration particles can produce recognizable changes without requiring successive waves infection. Aspects cell-killing actions T2 bacteriophage discussed.