Characterization of the exocellular polysaccharides from Azotobacter chroococcum
作者： Gregory L. Cote , Larry H. Krull
DOI: 10.1016/0008-6215(88)84030-8
关键词: Nuclear magnetic resonance spectroscopy 、 Acetal 、 Azotobacter chroococcum 、 Polysaccharide 、 Organic chemistry 、 Galactose 、 Mannose 、 Chromatography 、 Strain (chemistry) 、 Rhamnose 、 Chemistry
摘要: Abstract Azotobacter chroococcum produces appreciable amounts of exocellular polysaccharide. Produced by A. strain NRRL B-14341 are at least two distinct polysaccharides which can be separated ion-exchange chromatography on DEAE-Sephadex A-25. Exopolysaccharide I (EPS-I) consists rhamnose, mannose, and galactose in the molar ratios 1:2:2, along with a trace glucose. EPS-I also contains pyruvic acetal acetic ester groups. II (EPS-II) mainly mannuronic acid, much smaller proportions guluronic acid. Acetic groups present EPS-II. Methylation analysis reduced EPS-II shows it to (1→4)-linked, 13 C-nuclear magnetic resonance spectroscopy indicates that is (1→4)-β- d -mannuronan. evidently low-guluronate form alginate. Batch-to-batch variation was observed relative polysaccharides, acetyl contents EPS-I, degree acetylation
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