Dietary bisphenol A prevents ovarian degeneration and bone loss in female mice lacking the aromatase gene (Cyp19 )

摘要: We previously generated mice lacking aromatase activity by targeted disruption of Cyp19 (ArKO mice), and reported phenotypes the female mice, showing hemorrhage formation follicular depletion in ovary, diminution uterine size, bone loss. In present study, we examined influence dietary bisphenol A (BPA), a monomer used for production polycarbonate known to have estrogenic activity, on these ArKO mice. When were fed chow diets supplemented with 0.1% or 1% (w/w) BPA 5 months, they protected from ovarian degeneration, loss dose-dependent manner. Northern blot analyses RNA showed differences expression levels insulin-like growth factor (IGF)-I, IGF-I receptor, differentiation factor 9 morphogenetic protein 15 as compared those ovaries wild-type The restored BPA. uteri, progesterone receptor vascular endothelial mRNAs was diminished, contrast, had little effect ovarian, skeletal structures conclusion, effects reproductive tract well tissue evident adult These results suggest that mouse is an animal model suitable studying chemicals estrogen vivo.